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Bacterial Sortase Substrate I, FRET

Home/Catalog peptide/Bacterial Sortase Substrate I, FRET
Bacterial Sortase Substrate I, FRETAdmin2021-01-05T09:29:19+00:00
Product Name Bacterial Sortase Substrate I, FRET
Purity HPLC>95%
Description This 5-amino acid peptide is a sortase substrate, C-terminal sorting signal. Sortase cleaves surface proteins at the LPXTG motif and catalyzes the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Sortases are a family of Gram-positive transpeptidases responsible for anchoring surface protein virulence factors to the peptidoglycan cell wall layer. Cleavage of this FRET substrate by sortase reveals the fluorescent signal, Abs/Em = 340/490 nm.
Storage -20°C
References Mazmanian, S. et al. PNAS 99, 2293 (2002); Ilangovan, U. et al. PNAS 98, 6056 (2001), Ton-That, H. et al. PNAS 96, 12424 (1999); W. Navarre and O. Schneewind Microbiol. Mol. Biol. Rev. 63, 174 (1999).
Molecular Weight 1015.2
Sequence (One-Letter Code) DABCYL-LPETG-EDANS
Sequence (Three-Letter Code) DABCYL - Leu - Pro - Glu - Thr - Gly - EDANS

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