AFC (7-amino-4-trifluoromethylcoumarin)-derived caspase substrates are widely used for the fluorimetric detection of various caspase activities. Cleavage of AFC peptides by caspases generate strongly fluorescent AFC that is monitored fluorimetrically at 500-510 nm with excitation of 370-390 nm. Caspase-3 substrate with kcat/Km = 1.3 ×106 M-1s-1, Caspase-7 substrate with kcat/Km = 1.3 ×104 M-1s-1
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