Cathepsin D and E FRET SubstrateAdmin2021-01-16T10:14:17+00:00
Cathepsin D and E FRET Substrate
Cathepsins D and E obtained from the hepatopancreas (liver) of Japanese common squid (Todarodes pacificus) instead of B, H or L internal quenching fluorescent substrates (Ab / Em = 328/393 nm). Cleavage occurs at the Phe-Phe amide bond, resulting in increased fluorescence, and is used to screen cathepsin D and E inhibitors, and to determine the activity of cathepsin D and E in tissue cell extracts.
Yasuda, Y. et al. J. Biochem. 125, 1137 (1999).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
7 - Methoxycoumarin - 4 - Acetyl - Gly - Lys - Pro - Ile - Leu - Phe - Phe - Arg - Leu - Lys(Dnp) - D - Arg - NH2