The Staphylococcus aureus transpeptidase Sortase A (SrtA) anchors virulence and colonization-associated surface proteins to the cell wall. SrtA selectively recognizes a C-terminal LPXTG motif. SrtA readily reacts with its native substrate Abz-LPETG-Dap(DNP)-NH2, cleaving it and catalyzing the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Cleavage of this FRET substrate can be monitored at Ex/Em=320 nm/420 nm.
Shams, H. et al. J. Immunol. 173, 1966 (2004).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
H - Glu - Ile - Ser - Thr - Asn - Ile - Arg - Gln - Ala - Gly - Val - Gln - Tyr - Ser - Arg - OH