This is histone H3 (1-21), in which Arg2, Arg8 and Arg17 are deaminated to citrulline (Cit). Its C-terminus is biotinylated by the GGK linker and blocked by an amide group. Peptidyl arginine deaminase 4 (PADI4) prevents the methylation of these sites and blocks transcriptional activation. When the peptide purity is greater than 95%, the peptide is dissolved in distilled water at a concentration of 1 mg/ml, and lyophilized again into a powder form.
Cuthbert, G. et al. Cell 118, 545 (2004).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
H - Ala - Cit - Thr - Lys - Gln - Thr - Ala - Cit - Lys - Ser - Thr - Gly - Gly - Lys - Ala - Pro - Cit - Lys - Gln - Leu - Ala - Gly - Gly - Lys(Biotin) - NH2