Unlabeled phosphopeptide is an excellent substrate for mammalian protein tyrosine phosphatase 1B (Km=3.9µM) and Yersinia PTP. The sequence is derived from an autophosphorylation site of EGFR (Tyr992). Unlabeled peptides were used to determine PTB based on the significant fluorescence enhancement (Em=305 nm and Ex=282 nm) after removal of the phosphate group.
Ref: Ukkola, O. and MJ. Santaniemi, Intern. Med. 251, 467 (2002); Sarmiento, M. et al. J. Biol. Chem. 273, 26368 (1998); Zhang, ZY. et al. Anal. Biochem. 211, 7 (1993).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
Biotin - Asp - Ala - Asp - Glu - pTyr - Leu - Ile - Pro - Gln - Gln - Gly - OH