This is an N-terminal biotinylated peptide with phosphorylated Thr97. The sequence aprtpgrr contains a natural sequence derived from amino acids 95-98 of bovine myelin basic protein (PRTP). The rest of the sequence is not derived from the local sequence, but a synthetic structure. aprtpgrr is specific to MAP kinases: p44MAPK [extracellular signal-regulated kinase 1 (ERK1)] and p42MAPK (ERK2). It contains the consensus sequence Pro-X-(Ser/Thr)-Pro recognized by MAP kinase. aprtpgrr is the most effective substrate for the phosphorylation of ERK. It can be phosphorylated by the kinase on threonine 97 or by MAPK p38.
Ref: Sommer, F. et al. Int. J. Impot. Res. 14, 217 (2002); Lents, NH. et al. Ann. N. Y. Acad. Sci. 973, 265 (2002); Kameshita, I. et al. J. Biochem. (Tokyo) 122, 168 (1997).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
Biotin - Ala - Pro - Arg - pThr - Pro - Gly - Gly - Arg - Arg - OH