Gelatinase-2 (MMP-4) is used in combination with the developer (MMP-4) of the developer MMP-4-DITELLA-4 (MMP-4). The Km of this thiopeptidase reaction is 0.004 M, and the kcat is 103 s-1. Using this substrate, collagenase can be detected at a concentration as low as 2ng/mL. HPLC and tandem mass spectrometry are also used to analyze MMP-2 and MMP-9.
Ref: Weingarten, H. and J. Feder, Anal. Biochem. 147, 437 (1985); Weingarten, H. et al. Biochem. 24, 6730 (1985).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
Ac - Pro - Leu - Gly - SCH[CH2CH(CH3)2] - CO - Leu - Gly - OC2H5