NFF-3 is hydrolyzed quickly by MMP-3 (kcat/Km=218000 s-1 M-1), and hydrolyzed very slowly by MMP-9 (kcat/Km=10100 s-1 M-1), but MMP-1 and MMP -2 has no significant hydrolysis. It is one of the few synthetic substrates selectively hydrolyzed by some members of the MMP family, and therefore has important applications in distinguishing MMP-3 activity from other MMP activities. Abs/Em=325/393nm.
Ref: Bremer, C. et al. Acad. Radiol. 9 Suppl 2, S314 (2002); Nagase, H. et al. J. Biol. Chem. 269, 20952 (1994).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
Mca - Arg - Pro - Lys - Pro - Val - Glu - Nva - Trp - Arg - Lys(Dnp) - NH2