Monoclonal antibody is derived from one single B lymphocyte clone, only can bind with one epitope.
Because of monoclonal antibodies’ high-specificity, high-purity and high-homogeneity, they are widely used in enzyme-linked immunosorbent assay(ELISA), radioimmunoassay(RIA), immunohistochemistry and flow cytometry.
In anticancer therapy, monoclonal antibody has become a effective carrier for cancer drug targeting delivery; some monoclonal antibodies can bind with tumor cell surface protein, to inhibit protein expression; some monoclonal antibodies can induce immune response of B lymphocyte, induce the tumor cell apoptosis. By now, there are already several monoclonal antibodies be used in clinical like Rituximab, Trastuzumab, Cetuximab, etc.
B lymphocyte hybridoma technique is the production method of monoclonal antibody.