Monoclonal antibody is derived from one single B lymphocyte clone, only can bind with one epitope.

Because of monoclonal antibodies’ high-specificity, high-purity and high-homogeneity, they are widely used in enzyme-linked immunosorbent assay(ELISA), radioimmunoassay(RIA), immunohistochemistry and flow cytometry.

In anticancer therapy, monoclonal antibody has become a effective carrier for cancer drug targeting delivery; some monoclonal antibodies can bind with tumor cell surface protein, to inhibit protein expression; some monoclonal antibodies can induce immune response of B lymphocyte, induce the tumor cell apoptosis. By now, there are already several monoclonal antibodies be used in clinical like Rituximab, Trastuzumab, Cetuximab, etc.

B lymphocyte hybridoma technique is the production method of monoclonal antibody.

Hybridoma technique

Production steps:

  • Inject the antigen into animal for immunization, get sensitized lymphocytes. Get fusion of the sensitized lymphocytes and myeloma cells.
  • Cultivation in selective medium, select and grow the hybridoma cell. Then select the cells that can make desired antibody.
  • Let the selected cells grow in mass culture or be injected into animal to induce tumors.
  • Separate and purify the desired antibody from culture medium or animal ascites.