The peptide is histone H3 (15-36), asymmetrically dimethylated at Arg26, then C-terminal GGK, and biotinylated on the lysine side chain. Dimethylation on Arg26 is catalyzed by CARM1, and deamination is inhibited by peptidyl arginine deaminase (PADI)-4. Arginine methylation of histone H3 is related to transcription activation. When the peptide purity is greater than 95%, the peptide is dissolved in distilled water at a concentration of 1 mg/ml, and lyophilized again into a powder form.
Cuthbert, G. et al. Cell 118, 545 (2004).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
H - Ala - Pro - Arg - Lys - Gln - Leu - Ala - Thr - Lys - Ala - Ala - Arg(Me2a) - Lys - Ser - Ala - Pro - Ala - Thr - Gly - Gly - Val - Lys - Gly - Gly - Lys(Biotin) - OH