The peptide is histone H4 (1-23) asymmetrically dimethylated at Arg3, and then biotinylated lysine binds to the C-terminal GG linker. The methylation of Arg3 is catalyzed by PRMT1 and promotes the acetylation of histone H4 p300. Dimethylation of Arg3 also activates the transcription of estrogen-regulated genes. When the peptide purity is greater than 95%, the peptide is dissolved in distilled water at a concentration of 1 mg/ml, and lyophilized again into a powder form.
Arita, K. et al. Proc Natl Acad Sci ,b>103, 5291 (2006); Wang, H. et al. Science 293, 853 (2001).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
H - Ser - Gly - Arg(Me2a) - Gly - Lys - Gly - Gly - Lys - Gly - Leu - Gly - Lys - Gly - Gly - Ala - Lys - Arg - His - Arg - Lys - Val - Leu - Arg - Gly - Gly - Lys(Biotin) - OH