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Bacterial Sortase Substrate III, Abz/DNP

Home/Catalog peptide/Bacterial Sortase Substrate III, Abz/DNP
Bacterial Sortase Substrate III, Abz/DNPAdmin2021-01-05T09:34:52+00:00
Product Name Bacterial Sortase Substrate III, Abz/DNP
Purity HPLC>95%
Description The Staphylococcus aureus transpeptidase Sortase A (SrtA) anchors virulence and colonization-associated surface proteins to the cell wall. SrtA selectively recognizes a C-terminal LPXTG motif. SrtA readily reacts with its native substrate Abz-LPETG-Dap(DNP)-NH2, cleaving it and catalyzing the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Cleavage of this FRET substrate can be monitored at Ex/Em=320 nm/420 nm.
Storage -20°C
References Bentley, M. et al. J. Biol. Chem. 282, 6571 (2007).
Molecular Weight 928.0
Sequence (One-Letter Code) Abz-LPETG-K(Dnp)-NH2
Sequence (Three-Letter Code) Abz - Leu - Pro - Glu - Thr - Gly - Lys(Dnp) - NH2

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