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Calpain Substrate, Fluorogenic

Home/Catalog peptide/Calpain Substrate, Fluorogenic
Calpain Substrate, FluorogenicAdmin2021-01-07T09:12:34+00:00
Product Name Calpain Substrate, Fluorogenic
Purity HPLC>95%
Description A very sensitive fluorescent calpain substrate, Abs/Em=501/527 nm. In general, rh110-based protease substrates are more sensitive than similar AMC or afc substrates. This enhanced sensitivity may be due to the larger luciferase product. Colorless and non-fluorescent (Z-Ala-Ala) 2Rh110 is hydrolyzed into highly fluorescent rhodamine 110. The rhodamine 110 has excellent spectral characteristics and meets the best detection window of most fluorescent instruments. In addition, (Z-Ala-Ala)2Rh110 can also be used to detect calpain in the color development mode, because the enzyme product (Rhodamin 110) exhibits a large extinction coefficient (close to 100000 cm-1mol-1). After cleavage, Abs(max) is 497 nm, Em(max) is 527 nm
Storage -20°C
References Gitler D and Spira ME (1998). Real time imaging of calcium-induced localized proteolytic activity after axotomy and its relation to growth cone formation. Neuron 20, 1123-35, Rothe G, et al. (1992). Flow cytometric analysis of protease activities in vital cells. Biol Chem Hoppe Seyler 373, 547-54, Rothe G, et al. (1992). Flow cytometric analysis of protease activities in vital cells. Biol Chem Hoppe Seyler 373, 547-54.
Molecular Weight 885.6
Sequence (One-Letter Code) (Z-AA)2Rh110
Sequence (Three-Letter Code) (Z - Ala - Ala)2Rh110

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