The peptide is histone H3 (21-44), which is deaminated at Arg26 and converted to Cit (citrulline). It is biotinylated via a C-terminal GGK connector. Peptidyl arginine deaminase 4 (PADI4) blocks the methylation of CARM1 methyltransferase and inhibits transcription activation. When the peptide purity is greater than 95%, the peptide is dissolved in distilled water at a concentration of 1 mg/ml, and lyophilized again into a powder form.
Cuthbert, G. et al. Cell 118, 545 (2004).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
H - Ala - Thr - Lys - Ala - Ala - Cit - Lys - Ser - Ala - Pro - Ala - Thr - Gly - Gly - Val - Lys - Lys - Pro - His - Arg - Tyr - Arg - Pro - Gly - Gly - Gly - Lys(Biotin) - OH