Multiple antigen peptides (MAPs) are artificially branched peptides with Lys residues as the core of the scaffold, supporting the formation of branches with different or identical peptide sequences. It is a method for producing high-titer peptide antibodies and synthetic peptide vaccines. This system utilizes the α- and ε amino functional groups of lysine to form a main chain of polypeptide chains. Depending on the number of lysine layers (2, 4, 8, etc.), different numbers of peptide branches can be synthesized. This technique can successfully produce high-titer antibodies. MAPs have a high molar ratio of peptide antigen to core molecule and do not require carrier protein to induce antibody response.
1, Wang CY. et al. Science 254:285-288 (1991) 2, Posnett D. et al. J Biol. Chem. 263:1719-1725 (1988) 3, Tam JP. PNAS USA, 85:5409-5413 (1988) 4, Kowasczyk W. et al. J Pept Sci, 17(4):247-251 (2011).