This peptide is the c-terminal fragment of exendin-4. This structure with 20 residues folds more than 95% in water at physiological pH and presents a tightly folded tertiary structure in solution. Trp Cage is a highly stable microprotein folding body. It consists of a short helix, a 3, 10 helix and a c-terminal polyproline, which is combined with Trp in the alpha helix. It can be folded in 4 nanoseconds. This stable and fast-folding Trp Cage sequence shows the unique stability of this microprotein.
Ding, F. et al. Biophys. J. 88, 147 (2005); Neidigh, J. et al. Nat. Struct. Biol. 9, 425 (2002); Adams, C. et al. J. Am. Soc. Mass. Spectrom. 15, 1087 (2004); Herman, R. et al. J. Biol. Chem. 282, 9813 (2007).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
H - Asn - Leu - Tyr - Ile - Gln - Trp - Leu - Lys - Asp - Gly - Gly - Pro - Ser - Ser - Gly - Arg - Pro - Pro - Pro - Ser